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Irisin, recombinant (Human, Rat, Mouse, Canine) - ELISA Kit

Quantity:
Catalog #
Size
Price
EK-067-29 96 wells $500

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • 10-50 ng/ml
  • Sample Extraction:
  • Not required
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 1.29 ng/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 1.29 - 27.5 ng/ml
  • Cross Reactivity:
  • Peptide %
    Irisin, Recombinant (Human, Rat, Mouse, Canine) 100
    Irisin (42-112) (Human, Rat, Mouse, Canine) 100
    FNDC5, isoform 4 recombinant (Human, Rat, Mouse, Canine) 9
    Irisin precursor, C-terminal 48mer / FNDC5 (165-212) (Human) / FNDC5(162-209) (Rat, Mouse) 0
    Irisin (42-95) (Human, Rat, Mouse, Canine) 0
    FNDC4 (47-101) (Human) / FNDC4 (43-97) (Mouse) 0
    FNDC4 (103-158) (Human) / FNDC4 (99-154) (Mouse) 0
    FNDC4 / FRCP1 (190-234) (Human) 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
  • Storage:
  • Store at 4°C
Standard Curve:

Irisin: A Potential Link between Physical Exercise and Metabolism-An Observational Study in Differently Trained Subjects, from Elite Athletes to Sedentary People.
Benedini S, Dozio E, Invernizzi PL, et al. J Diabetes Res. 2017;2017:1039161.

Plasma Irisin Modestly Increases during Moderate and High-Intensity Afternoon Exercise in Obese Females.
Winn NC, Grunewald ZI, Liu Y, Heden TD, Nyhoff LM, Kanaley JA. PLoS ONE. 2017;12(1):e0170690.

Association of Serum Irisin Concentrations with Presence and Severity of Coronary Artery Disease.
Deng W. Med Sci Monit. 2016;22:4193-4197.

Effects of Body Weight Reduction on Serum Irisin and Metabolic Parameters in Obese Subjects.
Fukushima Y, Kurose S, Shinno H, et al. Diabetes Metab J. 2016;40(5):386-395.

Circulating Levels of Irisin in Hypopituitary and Normal Subjects.
Pena-Bello L, Pértega-Diaz S, Sangiao-Alvarellos S, et al. Luque RM, ed. PLoS ONE. 2016;11(7):e0160364.

High Serum Irisin Level as an Independent Predictor of Diabetes Mellitus: A Longitudinal Population-Based Study.
Huh JH, Ahn SV, Choi JH, Koh SB, Chung CH. Medicine (Baltimore). 2016;95(23):e3742.

Irisin Is Regulated by CAR in Liver and Is a Mediator of Hepatic Glucose and Lipid Metabolism.
Mo L, Shen J, Liu Q, et al. Mol Endocrinol. 2016;30(5):533-42.

Irisin modulates the association of interleukin-17A with the presence of non-proliferative diabetic retinopathy in patients with type 2 diabetes.
Wang C, Wang L, Liu J, et al. Endocrine. 2016;53(2):459-64.

Time-dependent irisin concentration changes in patients affected by overt hypothyroidism.
Zybek-kocik A, Sawicka-gutaj N, Wrotkowska E, Sowi?ski J, Rucha?a M. Endokrynol Pol. 2016;67(5):476-480.

Circulating Adipocyte Fatty Acid Binding Protein (FABP4) Levels Are Associated with Irisin in the Middle-Aged General Chinese Population.
Zhang S, Yang L, Chen P, et al. PLoS ONE. 2016;11(1):e0146605.

Effects and Molecular Mechanism of GST-Irisin on Lipolysis and Autocrine Function in 3T3-L1 Adipocytes.
Gao S, Li F, Li H, Huang Y, Liu Y, Chen Y. PLoS ONE. 2016;11(1):e0147480.

Irisin inhibits hepatic gluconeogenesis and increases glycogen synthesis via the PI3K/Akt pathway in type 2 diabetic mice and hepatocytes.
Liu TY, Shi CX, Gao R, et al. Clin Sci. 2015;129(10):839-50.

Betatrophin levels are increased in women with gestational diabetes mellitus compared to healthy pregnant controls.
Ebert T, Kralisch S, Wurst U, et al. Eur J Endocrinol. 2015;173(1):1-7.

Circulating irisin is lower in gestational diabetes mellitus.
Zhao L, Li J, Li ZL, Yang J, Li ML, Wang GL. Endocr J. 2015;62(10):921-6.

Irisin in blood increases transiently after single sessions of intense endurance exercise and heavy strength training.
Nygaard H, Slettaløkken G, Vegge G, et al. PLoS ONE. 2015;10(3):e0121367.

Associations of betatrophin levels with irisin in Chinese women with normal glucose tolerance.
Xie X, Gao T, Yang M, et al. Diabetol Metab Syndr. 2015;7:26.

Elevated circulating levels of irisin and the effect of metformin treatment in women with polycystic ovary syndrome.
Li M, Yang M, Zhou X, et al. J Clin Endocrinol Metab. 2015;100(4):1485-93.

Plasma irisin in runners and nonrunners: no favorable metabolic associations in humans.
Hew-butler T, Landis-piwowar K, Byrd G, et al. Physiol Rep. 2015;3(1)

Impact of supervised exercise rehabilitation on daily physical activity of cardiopulmonary patients.
Ramadi A, Stickland MK, Rodgers WM, Haennel RG. Heart Lung. 2015;44(1):9-14.

Complexity-Based Measures Inform Effects of Tai Chi Training on Standing Postural Control: Cross-Sectional and Randomized Trial Studies.
Wayne PM, Gow BJ, Costa MD, et al. PLoS ONE. 2014;9(12):e114731.

Thermogenic capacity is antagonistically regulated in classical brown and white subcutaneous fat depots by high fat diet and endurance training in rats: impact on whole-body energy expenditure.
Wu MV, Bikopoulos G, Hung S, Ceddia RB. J Biol Chem. 2014;289(49):34129-40.

Brown adipose tissue improves whole-body glucose homeostasis and insulin sensitivity in humans.
Chondronikola M, Volpi E, Børsheim E, et al. Diabetes. 2014;63(12):4089-99.

Irisin and FGF21 are cold-induced endocrine activators of brown fat function in humans.
Lee P, Linderman JD, Smith S, et al. Cell Metab. 2014;19(2):302-9.

The identification of irisin in human cerebrospinal fluid: influence of adiposity, metabolic markers, and gestational diabetes.
Piya MK, Harte AL, Sivakumar K, et al. Am J Physiol Endocrinol Metab. 2014;306(5):E512-8.

Serum levels of irisin in gestational diabetes mellitus during pregnancy and after delivery.
Ebert T, Stepan H, Schrey S, et al. Cytokine. 2014;65(2):153-8.

Plasma irisin depletion under energy restriction is associated with improvements in lipid profile in metabolic syndrome patients.
De la iglesia R, Lopez-legarrea P, Crujeiras AB, et al. Clin Endocrinol (Oxf). 2014;81(2):306-11.

Irisin and FNDC5 in retrospect: An exercise hormone or a transmembrane receptor?.
Erickson HP. Adipocyte. 2013;2(4):289-93.

Exercise training does not increase muscle FNDC5 protein or mRNA expression in pigs.
Fain JN, Company JM, Booth FW, et al. Metab Clin Exp. 2013;62(10):1503-11.

Resistance exercise training does not affect plasma irisin levels of hemodialysis patients.
Moraes C, Leal VO, Marinho SM, et al. Horm Metab Res. 2013;45(12):900-4.

Circulating irisin in relation to insulin resistance and the metabolic syndrome.
Park KH, Zaichenko L, Brinkoetter M, et al. J Clin Endocrinol Metab. 2013;98(12):4899-907.

Effects of obesity, diabetes and exercise on Fndc5 gene expression and irisin release in human skeletal muscle and adipose tissue: in vivo and in vitro studies.
Kurdiova T, Balaz M, Vician M, et al. J Physiol (Lond). 2014;592(5):1091-107.

The effects of acute and chronic exercise on PGC-1α, irisin and browning of subcutaneous adipose tissue in humans.
Norheim F, Langleite TM, Hjorth M, et al. FEBS J. 2014;281(3):739-49.