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Cholecystokinin (CCK) (26-33), non-sulfated (Human, Rat, Mouse, Porcine, Canine, Bovine, Chicken) - EIA Kit

Quantity:

Catalog #
Size
Price
EK-069-04 96 wells $570

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • Human Plasma: 0.13 ng/ml
    Human Plasma (extracted): 0.01 ng/ml
    Human Serum: 0.16 ng/ml
  • Sample Extraction:
  • Recommended
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 0.06 ng/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 0.06 - 0.85 ng/ml
  • Cross Reactivity:
  • Peptide %
    CCK (26-33) (Non-Sulfated) (Human, Rat, Mouse) 100
    CCK (26-33) (Sulfated) (Human, Rat, Mouse, Canine) 100
    CCK (27-33) (Human, Rat, Mouse) 100
    CCK-33 (Human) 100
    Big Gastrin-1 (Human) 100
    Gastrin-1 (Human) 100
    Caerulein 100
    CCK (30-33) (Human, Rat, Mouse)
    43
    CCK (1-21) (Human)
    0
    Pancreatic Polypeptide (Human) 0
    VIP (Human, Rat, Mouse, Porcine, Ovine) 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
Standard Curve:

Novel CCK-dependent vasorelaxing dipeptide, Arg-Phe, decreases blood pressure and food intake in rodents.
Kagebayashi T, Kontani N, Yamada Y, et al. Mol Nutr Food Res. 2012;56(9):1456-63.

The role of a pre-load beverage on gastric volume and food intake: comparison between non-caloric carbonated and non-carbonated beverage.
Cuomo R, Savarese MF, Sarnelli G, et al. Nutr J. 2011;10:114.

The G protein-coupled bile acid receptor, TGR5, stimulates gallbladder filling.
Li T, Holmstrom SR, Kir S, et al. Mol Endocrinol. 2011;25(6):1066-71.

Altered gene expressions of ghrelin, PYY, and CCK in the gastrointestinal tract of the hyperphagic intrauterine growth restriction rat offspring.
Nagata E, Nakagawa Y, Yamaguchi R, et al. Horm Metab Res. 2011;43(3):178-82.

Role of gut-brain axis in persistent abnormal feeding behavior in mice following eradication of Helicobacter pylori infection.
Bercik P, Verdú EF, Foster JA, et al. Am J Physiol Regul Integr Comp Physiol. 2009;296(3):R587-94.

SREBP-2 regulates gut peptide secretion through intestinal bitter taste receptor signaling in mice.
Jeon TI, Zhu B, Larson JL, Osborne TF. J Clin Invest. 2008;118(11):3693-700.

Calcium-sensing receptor mediates phenylalanine-induced cholecystokinin secretion in enteroendocrine STC-1 cells.
Hira T, Nakajima S, Eto Y, Hara H. FEBS J. 2008;275(18):4620-6.

Free fatty acids induce cholecystokinin secretion through GPR120.
Tanaka T, Katsuma S, Adachi T, Koshimizu TA, Hirasawa A, Tsujimoto G. Naunyn Schmiedebergs Arch Pharmacol. 2008;377(4-6):523-7.

Tachykinin neurokinin 3 receptor signaling in cholecystokinin-elicited release of oxytocin and vasopressin.
Haley GE, Flynn FW. Am J Physiol Regul Integr Comp Physiol. 2008;294(5):R1760-7.

Sustained weight loss after Roux-en-Y gastric bypass is characterized by down regulation of endocannabinoids and mitochondrial function.
Guijarro A, Osei-hyiaman D, Harvey-white J, et al. Ann Surg. 2008;247(5):779-90.

The effect of whole-body radiation on blood levels of gastrointestinal peptides in the rat.
Katanyutanon S, Wu R, Wang P. Int J Clin Exp Med. 2008;1(4):332-7.

Effects of active immunization against cholecystokinin 8 on performance, contents of serum hormones, and expressions of CCK gene and CCK receptor gene in pigs.
Zhang K, Yuan Z, Bing Y, Chen X, Ding X, Chen D. Endocrine. 2007;32(3):338-44.

Characterization of weight loss and weight regain mechanisms after Roux-en-Y gastric bypass in rats.
Guijarro A, Suzuki S, Chen C, et al. Am J Physiol Regul Integr Comp Physiol. 2007;293(4):R1474-89.

Enteroendocrine cells express functional Toll-like receptors.
Bogunovic M, Davé SH, Tilstra JS, et al. Am J Physiol Gastrointest Liver Physiol. 2007;292(6):G1770-83.

GPR93 activation by protein hydrolysate induces CCK transcription and secretion in STC-1 cells.
Choi S, Lee M, Shiu AL, Yo SJ, Halldén G, Aponte GW. Am J Physiol Gastrointest Liver Physiol. 2007;292(5):G1366-75.

Effects of acute and chronic protein intake on metabolism, appetite, and ghrelin during weight loss.
Leidy HJ, Mattes RD, Campbell WW. Obesity (Silver Spring). 2007;15(5):1215-25.

Effects of solid versus liquid meal-replacement products of similar energy content on hunger, satiety, and appetite-regulating hormones in older adults.
Tieken SM, Leidy HJ, Stull AJ, Mattes RD, Schuster RA, Campbell WW. Horm Metab Res. 2007;39(5):389-94.

Activation of enteroendocrine cells via TLRs induces hormone, chemokine, and defensin secretion.
Palazzo M, Balsari A, Rossini A, et al. J Immunol. 2007;178(7):4296-303.

Pork peptone stimulates cholecystokinin secretion from enteroendocrine cells and suppresses appetite in rats.
Sufian MK, Hira T, Miyashita K, Nishi T, Asano K, Hara H. Biosci Biotechnol Biochem. 2006;70(8):1869-74.

Dietary acetic acid reduces serum cholesterol and triacylglycerols in rats fed a cholesterol-rich diet.
Fushimi T, Suruga K, Oshima Y, Fukiharu M, Tsukamoto Y, Goda T. Br J Nutr. 2006;95(5):916-24.

Delayed postprandial gastric emptying and impaired gallbladder contraction together with elevated cholecystokinin and peptide YY serum levels sustain satiety and inhibit hunger in healthy elderly persons.
Di francesco V, Zamboni M, Dioli A, et al. J Gerontol A Biol Sci Med Sci. 2005;60(12):1581-5.

Changes in GI hormones and their effect on gastric emptying and transit times after Roux-en-Y gastric bypass in rat model.
Suzuki S, Ramos EJ, Goncalves CG, Chen C, Meguid MM. Surgery. 2005;138(2):283-90.

Delayed liquid gastric emptying in patients with hepatocellular carcinoma.
Chen CY, Lu CL, Chang FY, et al. Am J Gastroenterol. 2000;95(11):3230-7.

Effect of tropisetron on circulating catecholamines and other putative biochemical markers in serum of patients with fibromyalgia.
Höcherl K, Färber L, Ladenburger S, et al. Scand J Rheumatol Suppl. 2000;113:46-8.