Menu

ACTH (Human) - Fluorescent EIA Kit

Quantity:
Catalog #
Size
Price
FEK-001-01 96 wells $500

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • Human Plasma (extracted): 40.8 pg/ml
  • Sample Extraction:
  • Recommended
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 42.7 pg/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 42.7 - 1828 pg/ml
  • Cross Reactivity:
  • Peptide %
    ACTH (Human) 100
    ACTH (Rat, Mouse) 100
    ACTH (1-24) (Human) 100
    ACTH (7-38) (Human) 100
    β-Endorphin (Human) 0
    CRF (Human, Rat) 0
    α-MSH 0
    Met-Enkephalin 0
    α-ANP (1-28) (Human, Canine) 0
    BNP-32 (Human) 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
  • Absorption:
  • 325 nm
  • Max Emission:
  • 664 nm
Standard Curve:

Preliminary Plasma Levels

Sample Type Human Extracted*Plasma (Pooled)
Linear Range** 15.6-1691 pg/ml
ED50 88.23 pg/ml
Sample Level 40.8 pg/ml

*Extraction was performed on human plasma as described in the extraction protocol.

**Linear Range varies by lot.

Note:Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.