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Agouti-Related Peptide (AgRP) (71-132), Form C amide (Human) - Fluorescent EIA Kit

Quantity:

Catalog #
Size
Price
FEK-003-55 96 wells $518

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • Human Plasma (extracted): 11 pg/ml
  • Sample Extraction:
  • Recommended
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 28.4 pg/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 28.4 - 298 pg/ml
  • Cross Reactivity:
  • Peptide %
    Agouti-Related Protein Form C-NH2 (Human) 100
    Agouti-Related Protein (83-132)-NH2 (Human) 100
    Agouti-Related Protein (82-131)- NH2 (Mouse) 100
    Leptin (Human) 0
    Orexin A (Human, Mouse, Rat) 0
    Orexin B (Human) 0
    Neuropeptide Y (Human, Rat) 0
    α-MSH 0
    MCH (Human, Mouse, Rat) 0
    CGRP (Rat) 0
    CGRP (Human) 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
  • Absorption:
  • 325 nm
  • Max Emission:
  • 420 nm
Standard Curve:

Preliminary Plasma Levels

Sample Type Human Extracted*Plasma (Pooled)
Linear Range** 57-575 pg/ml
ED50 135.07 pg/ml
Sample Level 11 pg/ml

*Extraction was performed on human plasma as described in the extraction protocol.

**Linear Range varies by lot.

Note:Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.