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ANP, alpha (1-28) (Human, Porcine, Ovine, Canine) - Fluorescent EIA Kit

Quantity:

Catalog #
Size
Price
FEK-005-06 96 wells $518

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • Human Plasma (extracted): 35.8 pg/ml
  • Sample Extraction:
  • Recommended
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 14.8 pg/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 14.8 - 338 pg/ml
  • Cross Reactivity:
  • Peptide %
    alpha-ANP (1-28) (Human, Ovine, Canine) 100
    beta-ANP (Human) 100
    ANP (Rat) 0
    BNP-26 (Porcine) <0.1
    Endothelin-1 (Human, Rat, Porcine,Mouse, Canine, Bovine) 0
    BNP-32 (Human) 0
    BNP-45 (Rat) 0
    CNP-22 (Human, Porcine, Rat) 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
  • Absorption:
  • 325 nm
  • Max Emission:
  • 420 nm
Standard Curve:

Preliminary Plasma Levels

Sample Type Human Extracted*Plasma (Pooled)
Linear Range** 25.4-558 pg/ml
ED50 95.94 pg/ml
Sample Level 35.84 pg/ml

***Note:Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.