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Adrenomedullin (Human) - Fluorescent EIA Kit

Quantity:

Catalog #
Size
Price
FEK-010-01 96 wells $533

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • Human Plasma (extracted): 103 pg/ml
  • Sample Extraction:
  • Recommended
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 31.6 pg/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 31.6 - 428 pg/ml
  • Cross Reactivity:
  • Peptide %
    Adrenomedullin (Human) 100
    Adrenomedullin (Rat) 100
    Adrenomedullin (13-52) (Human)
    100
    Adrenomedullin (1-50) (Mouse) 100
    Adrenomedullin-Gly (Human)
    <1
    Pro-ADM N20 (Human) 0
    Pro-ADM N20 (Rat) 0
    Amylin (Human) 0
    CGRP (Human)
    0
    CGRP (Human) 0
    Endothelin-1 (ET-1) (Human, Bovine, Canine, Mouse, Porcine, Rat) 0
    α-ANP (1-28) (Human, Canine, Ovine, Porcine) 0
    BNP-32 (Human)
    0
    CNP-22 (Human, Rat, Mouse, Porcine)
    0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
  • Absorption:
  • 325 nm
  • Max Emission:
  • 420 nm
Standard Curve:

Preliminary Plasma Levels

Sample Type Human Extracted*Plasma (Pooled)
Linear Range** 110-733 pg/ml
ED50 215.9 pg/ml
Sample Level 103 pg/ml

*Extraction was performed on human plasma as described in the extraction protocol.

**Linear Range varies by lot.

Note:Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.