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BNP-32 (Human) - Fluorescent EIA Kit

Quantity:

Catalog #
Size
Price
FEK-011-03 96 wells $508

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • Human Plasma (extracted): 53.7 pg/ml
  • Sample Extraction:
  • Recommended
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 14.9 pg/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 14.9 - 589 pg/ml
  • Cross Reactivity:
  • Peptide %
    BNP-32 (Human) 100
    BNP-32 (Rat) <0.04
    BNP-45 (Rat) 0
    BNP-26 (Porcine) 0
    α-ANP (1-28) (Human, Canine) 0
    Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine) 0
    Angiotensin II (Human) 0
    VIP (Human, Rat, Porcine) 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
  • Absorption:
  • 325 nm
  • Max Emission:
  • 420 nm
Standard Curve:

Preliminary Plasma Levels

Sample Type Human Extracted*Plasma (Pooled)
Linear Range** 46.6-1681 pg/ml
ED50 203.6 pg/ml
Sample Level 53.7 pg/ml

***Note:Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples