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Orphanin FQ / Nociceptin (Human, Rat, Mouse, Ox) - Fluorescent EIA Kit

Quantity:

Catalog #
Size
Price
FEK-021-55 96 wells $624

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • Human Plasm (extracted): 31.4 pg/ml
  • Sample Extraction:
  • Recommended
  • Cross Reactivity:
  • Peptide %
    Orphanin FQ (OFQ) 100
    Met-Enkephalin 0
    Dynorphin A 0
    β-Endorphin (Human) 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
  • Absorption:
  • 325 nm
  • Max Emission:
  • 420 nm
Standard Curve:

Preliminary Plasma Levels

Sample Type Human Extracted*Plasma (Pooled)
Linear Range** 27.7-471 pg/ml
ED50 78.8 pg/ml
Sample Level 31.4 pg/ml

*Extraction was performed on human plasma as described in the extraction protocol.

**Linear Range varies by lot.

Note:Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.