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Apelin-12 (Human, Rat, Mouse, Bovine) - Fluorescent EIA Kit

Quantity:
Catalog #
Size
Price
FEK-057-23 96 wells $500

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • Human Plasma (extracted): 9 pg/ml
  • Sample Extraction:
  • Recommended
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 15.8 pg/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 15.8 - 419 pg/ml
  • Cross Reactivity:
  • Peptide %
    Apelin-12 (Human) 100
    Apelin-13 (Human) 100
    Apelin-13 (Rat) 100
    Apelin-36 (Human) 100
    Apelin-36 (Rat) 100
    Alpha-ANP (Human) 0
    ANP (25-56) (N-ANP) 0
    ADM-52 (Human) 0
    BNP-32 (Human) 0
    CNP-22 0
    Ghrelin (Human) 0
    ET-1 (Human) 0
    Bradykinin 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
  • Absorption:
  • 325 nm
  • Max Emission:
  • 420 nm
Standard Curve:
Pyroglutamyl apelin-13 identified as the major apelin isoform in human plasma.
Zhen EY, Higgs RE, Gutierrez JA. Anal Biochem. 2013;442(1):1-9.