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VIP (Human, Rat, Mouse, Porcine, Ovine) - Fluorescent EIA Kit

Quantity:

Catalog #
Size
Price
FEK-064-16 96 wells $518

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • Human Plasma (extracted): 13.5 pg/ml
  • Sample Extraction:
  • Recommended
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 13.3 pg/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 13.3 - 301 pg/ml
  • Cross Reactivity:
  • Peptide %
    VIP (Human, Rat, Mouse, Porcine, Ovine) 100
    VIP (Guinea Pig) 100
    VIP (10-28) (Human, Porcine, Rat) 34.6
    VIP (Chicken) 28
    PACAP-27-NH2 (Human, Rat, Ovine) <0.02
    VIP (1-12) (Human, Porcine, Rat) 0
    PHM-27 (Human) 0
    Substance P 0
    Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine) 0
    Secretin (Porcine) 0
    Glucagon (Human) 0
    Galanin (Rat) 0
    Somatostatin 0
    PACAP-38 (Human, Rat, Ovine) 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
  • Absorption:
  • 325 nm
  • Max Emission:
  • 420 nm
  • MSDS:
  • View/Download(PDF)
Standard Curve:

Preliminary Plasma Levels

Sample Type  Human Extracted* Plasma (Pooled)
Linear Range ** 33.2-434 pg/ml
ED50  96.03 pg/ml
Sample Level 13.5 pg/ml

* Extraction was performed on human plasma as described in the extraction protocol.

** Linear Range varies by lot. 

Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.