- Protocol:
|
View/Download (PDF)
- for reference only
|
- Sample Preparation:
| Suggested Procedure |
- Measured Sample Levels:
Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results. | Human Plasma (extracted): 9.3 pg/ml
|
- Sample Extraction:
| Recommended |
- Sensitivity:
Data may differ slightly based on lot. | 8.4 pg/ml |
- Linear Range:
Data may differ slightly based on lot. | 8.4 - 843 pg/ml |
- Cross Reactivity:
Peptide |
% |
Urotensin II (Human) |
100 |
Urotensin II(5-11) (Human) |
22 |
Urotensin II (Rat) |
18.2 |
Urotensin II Related Peptide (Human,Mouse, Rat) |
15.7 |
Urotensin II (Mouse) |
15.1 |
Urotentin II (4-11) (Human) |
15 |
Urotensin II (102-125)-Prepro(Human) |
0 |
Urotensin I (Catostomus commersoni) |
0 |
Endothelin-1 |
0 |
Angiotensin II |
0 |
ADM (Rat) |
0 |
PAMP-20 (Human) |
0 |
a-ANP-28 (Human) |
0 |
CGRP (Rat) |
0 |
CNP-22 |
0 |
BNP-25 (Rat) |
0 |
BNP-32 (Rat) |
0 |
Bradykinin |
0 |
Neurotensin |
0 |
|
- Intra-assay variation:
| <10% |
- Inter-assay variation:
| <15% |
- Absorption:
| 325 nm |
- Max Emission:
|
420 nm |
Standard Curve:
Preliminary Plasma Levels
Sample Type |
Human Extracted* Plasma (Pooled) |
Linear Range ** |
8.4-843 pg/ml |
ED50 |
70.08 pg/ml |
Sample Level |
9.3 pg/ml |
* Extraction was performed on human plasma as described in the extraction protocol.** Linear Range varies by lot. Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples
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