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Bradykinin and Related Peptides

bradykinin sequence
Prekallikrein (PK) activation on human umbilical endothelial cells (HUVEC) presumably leads to bradykinin liberation. On HUVEC, PK activation requires the presence of cell-bound high-molecular-weight kininogen (HK) and Zn(2+). We examined the Zn(2+) requirement for HK binding to and the consequences of PK activation on endothelial cells. Optimal HK binding (14 pmol/10(6) HUVEC) is seen with no added Zn(2+) in HEPES-Tyrode buffer containing gelatin versus 16--32 microM added Zn(2+) in the same buffer containing bovine serum albumin. The affinity and number of HK binding sites on HUVEC are a dissociation constant of 9.6 +/- 1.8 nM and a maximal binding of 1.08 +/- 0.26 x 10(7) sites/cell (means +/- SD). PK is activated to kallikrein by an antipain-sensitive mechanism in the presence of HK and Zn(2+) on HUVEC, human microvascular endothelial cells, umbilical artery smooth muscle cells, and bovine pulmonary artery endothelial cells. Simultaneous with kallikrein formation, bradykinin (5.0 or 10.3 pmol/10(6) HUVEC in the absence or presence of lisinopril, respectively) is liberated from cell-bound HK. Liberated bradykinin stimulates the endothelial cell bradykinin B2 receptor to form nitric oxide. Assembly and activation of PK on endothelial cells modulates their physiological activities.

This publication used a Bradykinin peptide from Phoenix Pharmaceuticals.
Zhao Y, Qiu Q, Mahdi F, Shariat-madar Z, Røjkjaer R, Schmaier AH. Assembly and activation of HK-PK complex on endothelial cells results in bradykinin liberation and NO formation. Am J Physiol Heart Circ Physiol. 2001;280(4):H1821-9.



Inhibitory activity of blomhotins and potentiators against angiotensin-covering enzyme



Related Products

Catalog# Product Standard Size Price
EK-009-01 Bradykinin (Human, Rat, Mouse) - EIA Kit 96 wells $458
009-01 Bradykinin (Human, Rat, Mouse) 5 mg $21
RK-009-01 Bradykinin (Human, Rat, Mouse) - RIA Kit 125 tubes $588
FEK-009-01 Bradykinin (Human, Rat, Mouse) - Fluorescent EIA Kit 96 wells $500
009-18 Bradykinin / HOE 140 (Human, Rat, Mouse) 200 µg $97
009-37 Bradykinin / Kallidin (Human, Rat, Mouse) 500 µg $66
EK-009-01CE Bradykinin (Human, Rat, Mouse) - EIA Kit, CE Mark Certified 96 wells $478
FEK-009-01CE Bradykinin (Human, Rat, Mouse) - Fluorescent EIA Kit, CE Mark Certified 96 wells $520
009-02 Bradykinin (Human, Rat, Mouse) 500 µg $97
009-04 Bradykinin (Human, Rat, Mouse) 5 mg $87
009-05 Bradykinin (Human, Rat, Mouse) 500 µg $28
009-06 Bradykinin (Human, Rat, Mouse) 500 µg $97
009-07 Bradykinin (Human, Rat, Mouse) 1 mg $77
009-09 Bradykinin (Human, Rat, Mouse) 5 mg $61
009-10 Bradykinin (Human, Rat, Mouse) 500 µg $49
009-11 Bradykinin (Human, Rat, Mouse) 500 µg $49
009-12 Bradykinin (Human, Rat, Mouse) 100 µg $194
009-13 Bradykinin (Human, Rat, Mouse) 500 µg $66
009-15 Bradykinin (Human, Rat, Mouse) 500 µg $61
009-16 Bradykinin (Human, Rat, Mouse) 500 µg $66
009-17 Bradykinin (Human, Rat, Mouse) 200 µg $66
009-19 Bradykinin (Human, Rat, Mouse) 200 µg $66
009-20 Bradykinin (Human, Rat, Mouse) 200 µg $107
009-21 Bradykinin (Human, Rat, Mouse) 200 µg $122
009-22 Bradykinin (Human, Rat, Mouse) 200 µg $122
009-28 Bradykinin (Human, Rat, Mouse) 5 mg $56
009-33 Bradykinin (Human, Rat, Mouse) 500 µg $110
009-34 Bradykinin (Human, Rat, Mouse) 500 µg $97
009-35 Bradykinin (Human, Rat, Mouse) 200 µg $133
H-009-01 Bradykinin (Human, Rat, Mouse) - Antibody 50 µl $179

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