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Bradykinin and Related Peptides

bradykinin sequence
Prekallikrein (PK) activation on human umbilical endothelial cells (HUVEC) presumably leads to bradykinin liberation. On HUVEC, PK activation requires the presence of cell-bound high-molecular-weight kininogen (HK) and Zn(2+). We examined the Zn(2+) requirement for HK binding to and the consequences of PK activation on endothelial cells. Optimal HK binding (14 pmol/10(6) HUVEC) is seen with no added Zn(2+) in HEPES-Tyrode buffer containing gelatin versus 16--32 microM added Zn(2+) in the same buffer containing bovine serum albumin. The affinity and number of HK binding sites on HUVEC are a dissociation constant of 9.6 +/- 1.8 nM and a maximal binding of 1.08 +/- 0.26 x 10(7) sites/cell (means +/- SD). PK is activated to kallikrein by an antipain-sensitive mechanism in the presence of HK and Zn(2+) on HUVEC, human microvascular endothelial cells, umbilical artery smooth muscle cells, and bovine pulmonary artery endothelial cells. Simultaneous with kallikrein formation, bradykinin (5.0 or 10.3 pmol/10(6) HUVEC in the absence or presence of lisinopril, respectively) is liberated from cell-bound HK. Liberated bradykinin stimulates the endothelial cell bradykinin B2 receptor to form nitric oxide. Assembly and activation of PK on endothelial cells modulates their physiological activities.

This publication used a Bradykinin peptide from Phoenix Pharmaceuticals.
Zhao Y, Qiu Q, Mahdi F, Shariat-madar Z, Røjkjaer R, Schmaier AH. Assembly and activation of HK-PK complex on endothelial cells results in bradykinin liberation and NO formation. Am J Physiol Heart Circ Physiol. 2001;280(4):H1821-9.



Inhibitory activity of blomhotins and potentiators against angiotensin-covering enzyme



Related Products

Catalog# Product Standard Size Price
EK-009-01 Bradykinin (Human, Rat, Mouse) - EIA Kit 96 wells $465
009-01 Bradykinin (Human, Rat, Mouse) 5 mg $21
RK-009-01 Bradykinin (Human, Rat, Mouse) - RIA Kit 125 tubes $597
FEK-009-01 Bradykinin (Human, Rat, Mouse) - Fluorescent EIA Kit 96 wells $508
009-18 [D-Arg0, Hyp3, Thi5, D-Tic7, Oic8]-Bradykinin / HOE 140 (Human, Rat, Mouse) 200 µg $98
009-37 [Lys0]-Bradykinin / Kallidin (Human, Rat, Mouse) 500 µg $67
EK-009-01CE Bradykinin (Human, Rat, Mouse) - EIA Kit, CE Mark Certified 96 wells $485
FEK-009-01CE Bradykinin (Human, Rat, Mouse) - Fluorescent EIA Kit, CE Mark Certified 96 wells $528
009-02 [Sar0, D-Phe8, Des-Arg9]-Bradykinin (Human, Rat, Mouse) 500 µg $98
009-04 [Des-Pro2]-Bradykinin (Human, Rat, Mouse) 5 mg $88
009-05 [Hyp3]-Bradykinin (Human, Rat, Mouse) 500 µg $28
009-06 [Lys0, D-Phe8, Des-Arg9]-Bradykinin (Human, Rat, Mouse) 500 µg $98
009-07 [Ac-Lys0, (β-D-Nal7, Ile8, Des-Arg9]-Bradykinin (Human, Rat, Mouse) 1 mg $78
009-09 [Des-Arg9, Leu8]-Bradykinin (Human, Rat, Mouse) 5 mg $62
009-10 [Thi5, D-Phe7, Thi8]-Bradykinin (Human, Rat, Mouse) 500 µg $50
009-11 [D-Arg0, Hyp3, D-Phe7]-Bradykinin (Human, Rat, Mouse) 500 µg $50
009-12 [Tyr8]-Bradykinin (Human, Rat, Mouse) 100 µg $197
009-13 [D-Arg0, Hyp3, Thi5, D-Phe7, Thi8]-Bradykinin (Human, Rat, Mouse) 500 µg $67
009-15 [D-Arg0, Hyp2, Hyp3, Thi5, D-Phe7, Thi8]-Bradykinin (Human, Rat, Mouse) 500 µg $62
009-16 [Lys-Lys-Hyp3, Thi5, D-Phe7, Thi8]-Bradykinin (Human, Rat, Mouse) 500 µg $67
009-17 [D-Arg0, Hyp3, D-Tic7, Oic8]-Bradykinin (Human, Rat, Mouse) 200 µg $67
009-19 [Arg(Tos)1, Hyp3, Thi5, D-Tic7, Oic8]-Bradykinin (Human, Rat, Mouse) 200 µg $67
009-20 [Lys-Lys0, Hyp3, Igl5, D-Igl7, Oic8, Des-Arg9]-Bradykinin (Human, Rat, Mouse) 200 µg $109
009-21 [D-Arg0, Hyp3, Igl5, D-Igl7, Oic8, Des-Arg9]-Bradykinin (Human, Rat, Mouse) 200 µg $124
009-22 [D-Arg0, Hyp3, Igl5, D-Igl7, Oic8]-Bradykinin (Human, Rat, Mouse) 200 µg $124
009-28 [Mey-Lys0]-Bradykinin (Human, Rat, Mouse) 5 mg $57
009-33 [Ac-Lys0, Me-Ala6, Leu8, Des-Arg9]-Bradykinin (Human, Rat, Mouse) 500 µg $112
009-34 [Lys0, Leu8, Des-Arg9]-Bradykinin (Human, Rat, Mouse) 500 µg $98
009-35 [Lys-Lys0, Δ-Pro9, Hyp3, Igl5, D-Igl7, Oic8, Des-Arg9]-Bradykinin (Human, Rat, Mouse) 200 µg $135
H-009-01 Bradykinin (Human, Rat, Mouse) - Antibody 50 µl $182

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