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Magnetic bead conjugated to antibody

To enable fast, simple, and efficient purification of target antigens, we provide highly sensitive magnetic bead conjugated antibodies. Using these MagBeads, you no longer need to centrifuge and stress your samples. Additionally, our MagBeads and the target antigens they bind are very easy to separate and isolate from the rest of your sample. The high binding capacity, specificity of binding, and easy use is ideal for your immunoprecipitation, protein / peptide purification, and mass spectrometry studies.

Advantages of our MagBeads include:
- reproducible results (inter-assay and intra-assay variation < 10%)
- gentle on samples, minimizing protein / peptide degradation
- can be used with very small sample volumes (> 5 ul)
- versatile across sample types (e.g. plasma, CSF, tissue homogenate, cell culture)
- high recovery of target antigen (> 75%)

principle of mass spec

Our magnetic bead linked antibodies have already been used in a variety of applications. These include...
(1) Validation using small sample volumes (e.g. for CSF, tears, saliva)
(2) Quantification using mass spectrometry
(3) Quantification via magnetic bead assay

Diagrams and references of each validated application can be found below.


 

(1) Validation using small sample volumes (e.g. CSF, tears, saliva)

 

small sample analysis using magbead

 

References:

Escobar H, Kushnir MM, Rockwood AL, Meikle AW. High Sensitivity Measurement of Pancreatic Polypeptide and Its Variant in Serum and Plasma by LC-MS/MS. Methods Mol Biol. 2016;1378:199-210.
Cowan A, Lyu RM, Chen YH, Dun SL, Chang JK, Dun NJ. Phoenixin: A candidate pruritogen in the mouse. Neuroscience. 2015;310:541-8.
Yosten GL, Lyu RM, Hsueh AJ, et al. A novel reproductive peptide, phoenixin. J Neuroendocrinol. 2013;25(2):206-15.
Lyu RM, Huang XF, Zhang Y, et al. Phoenixin: a novel peptide in rodent sensory ganglia. Neuroscience. 2013;250:622-31.
Samson WK, Zhang JV, Avsian-kretchmer O, et al. Neuronostatin encoded by the somatostatin gene regulates neuronal, cardiovascular, and metabolic functions. J Biol Chem. 2008;283(46):31949-59.

 

(2) Quantification using mass spectrometry

 

maldi-tof

magbead mass spec bnp-32standard curve for BNP-32 derived from nano-LC-ESI-FT-ICR MS data

a) Chemical structure of stable isotope-labeled BNP-32 (BNP-32*). 

b) Standard curve for BNP-32 using data derived from nano-LC-ESI-FT-ICR MS data.

Figure from:  Adam M. Hawkridge et al. PNAS 2005;102:17442-17447

 

 

(3) Quantification via magnetic bead assay 

magbead immunoassay

References:

Stephanie Feldman  Robert Buchanan  William Carpenter et al., SU17. Oxytocin and Sexual Function in Males and Females With Schizophrenia. Schizophrenia Bulletin, Volume 43, Issue suppl_1, 1 March 2017, Pages S167
Strauss GP, Keller WR, Koenig JI, Gold JM, Frost KH, Buchanan RW. Plasma oxytocin levels predict social cue recognition in individuals with schizophrenia. Schizophr Res. 2015;162(1-3):47-51.
Strauss GP, Keller WR, Koenig JI, Sullivan SK, Gold JM, Buchanan RW. Endogenous oxytocin levels are associated with the perception of emotion in dynamic body expressions in schizophrenia. Schizophr Res. 2015;162(1-3):52-6.
Rocha EP, Danchin A. Essentiality, not expressiveness, drives gene-strand bias in bacteria. Nat Genet. 2003;34(4):377-8.
Kalamatianos T, Markianos M, Margetis K, Bourlogiannis F, Stranjalis G. Higher Orexin A levels in lumbar compared to ventricular CSF: a study in idiopathic normal pressure hydrocephalus. Peptides. 2014;51:1-3.
Cabiati M, Raucci S, Liistro T, et al. Impact of obesity on the expression profile of natriuretic peptide system in a rat experimental model. PLoS ONE. 2013;8(8):e72959.
Jawiarczyk-przyby?owska A, Bolanowski M. The role of orexin A in metabolic disturbances in patients with acromegaly. Endokrynol Pol. 2012;63(6):463-9.
Del ry S, Cabiati M, Vozzi F, et al. Expression of C-type natriuretic peptide and its receptor NPR-B in cardiomyocytes. Peptides. 2011;32(8):1713-8.