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Calcitonin increases bone calcium content and decrease the blood calcium level

schematic calcitonin schematic sequence calcitonin

schematic sequence comparison calcitonin

The most common cause of hereditary nephrogenic diabetes insipidus is a nonfunctional vasopressin (VP) receptor type 2 (V2R). Calcitonin, another ligand of G-protein–coupled receptors, has a VP-like effect on electrolytes and water reabsorption, suggesting that it may affect AQP2 trafficking. Here, calcitonin increased intracellular cAMP and stimulated the membrane accumulation of AQP2 in LLC-PK1 cells. Pharmacologic inhibition of protein kinase A (PKA) and deficiency of a critical PKA phosphorylation site on AQP2 both prevented calcitonin-induced membrane accumulation of AQP2. Fluorescence assays showed that calcitonin led to a 70% increase in exocytosis and a 20% decrease in endocytosis of AQP2. Immunostaining of rat kidney slices demonstrated that calcitonin induced a significant redistribution of AQP2 to the apical membrane of principal cells in cortical collecting ducts and connecting segments but not in the inner stripe or inner medulla. Calcitonin-treated VP-deficient Brattleboro rats had a reduced urine flow and two-fold higher urine osmolality during the first 12 hours of treatment compared with control groups. Although this VP-like effect of calcitonin diminished over the following 72 hours, the tachyphylaxis was reversible. Taken together, these data show that calcitonin induces cAMP-dependent AQP2 trafficking in cortical collecting and connecting tubules in parallel with an increase in urine concentration. This suggests that calcitonin has a potential therapeutic use in nephrogenic diabetes insipidus.

Appleyard SM, Hayward M, Young JI, et al. A role for the endogenous opioid beta-endorphin in energy homeostasis. Endocrinology. 2003;144(5):1753-60.


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Related Products

Catalog# Product Standard Size Price
EK-014-09 Calcitonin (Salmon) - EIA Kit 96 wells $465
014-06 Calcitonin (Rat) 500 µg $67
EK-014-06 Calcitonin (Rat) - EIA Kit 96 wells $465
014-22 Calcitonin (Mouse) 100 µg $124
EK-014-03 Calcitonin (Human, Monkey) - EIA Kit 96 wells $465
RK-014-06 Calcitonin (Rat) - RIA Kit 125 tubes $597
014-03 Calcitonin (Human, Monkey) 500 µg $93
EK-014-03CE Calcitonin (Human, Monkey) - EIA Kit, CE Mark Certified 96 wells $485
010-66 C7 / Calcitonin Receptor Antagonist 100 µg $187
014-20 Calcitonin (8-32) (Salmon) 200 µg $160
FC3-014-20 Calcitonin (8-32) (Salmon) - Cy3 Labeled 1 nmol $259
FC5-014-20 Calcitonin (8-32) (Salmon) - Cy5 Labeled 1 nmol $259
FG-014-20A Calcitonin (8-32) (Salmon) - FAM Labeled 1 nmol $207
FR-014-20 Calcitonin (8-32) (Salmon) - Rhodamine Labeled 1 nmol $207
014-01 Calcitonin (Chicken) 500 µg $88
014-02 Calcitonin (Eel) 500 µg $98
014-10 [Asu1, Asu7]-Calcitonin (Eel) 200 µg $88
H-014-02 Calcitonin (Eel) - Antibody 50 µl $182
T-014-02 Calcitonin (Eel) - I-125 Labeled 10 µCi $734
T-G-014-02 Calcitonin (Eel) - I-125 Labeled Purified IgG 10 µCi $734
G-014-02 Calcitonin (Eel) - Purified IgG Antibody 400 µg $337
RK-014-02 Calcitonin (Eel) - RIA Kit 125 tubes $597
014-08 [Met(O)8]-Calcitonin (Human) 100 µg $187
H-014-03 Calcitonin (Human, Monkey) - Antibody 50 µl $182
B-014-03 Calcitonin (Human, Monkey) - Biotin Labeled 100 µg $259
B-014-04 [epsilon-Biotinyl-Lys]-Calcitonin (Human, Monkey) - Biotin Labeled 100 µg $259
FG-014-03A Calcitonin (Human, Monkey) - FAM Labeled 1 nmol $207
FEK-014-03 Calcitonin (Human, Monkey) - Fluorescent EIA Kit 96 wells $508
FEK-014-03CE Calcitonin (Human, Monkey) - Fluorescent EIA Kit, CE Mark Certified 96 wells $528
T-014-03 Calcitonin (Human, Monkey) - I-125 Labeled 10 µCi $734

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