Tissue Sample |
Rat brain tissue homogenate |
Electrophoresis |
10% SDS-PAGE gel |
Negative control |
No primary antibody |
Blocking |
1% BSA in TBST or 5% dry milk in TBST |
Primary Antibody |
Rabbit Anti-GPR103/ AQ27 (401-433) (Human) Antiserum (Catalog No.:H-001-81) |
Optimal Dilution |
1:500 (4 hour at RT, or overnight at 4′ C) |
Secondary Antibody |
Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min) |
Detection system |
HRP |
Substrate |
SuperSignal Chemiluminescent Substrate |