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| Sequence: | Human Leptin is 146 Amino Acid residues protein cloned, expressed in E.Coli and purified. |
| Molecular Weight: | ~ 16 kDa |
| Purity: | ≥ 97% (determined by SDS-PAGE) |
| Validation: | Exhibits correct molecular weight |
| Solubility: | Insoluble in water. Dissolve at 2 mg/ml in 15 mM HCl. After protein is completely dissolved, add 7.5 mM NaOh to bring pH up to ~5.2. |
| Storage: | Store in dry form at -20°C to -70°C. For best results, rehydrate just before use. |
| Appearance: | White powder |
| Contents: | Each vial contains 200 µg of protein. |
| Intra-Assay CV: | <10% |
| Inter-Assay CV: | <15% |
| Sample Extraction: | |
| Standard Curve: |