ACTH (Rat, Mouse) - EIA Kit


Catalog #
EK-001-21 96 wells $570

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Sample Extraction:
  • Recommended
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 0.08 ng/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 0.08 - 1.78 ng/ml
  • Cross Reactivity:
  • Peptide %
    ACTH (Rat, Mouse) 100
    ACTH (Human) 100
    ACTH (18-39) (Human) 100
    ACTH (1-24) (Human) 0.01
    ACTH (7-38) (Human) 0
    ß-Endorphin (Rat) 0
    α-MSH 0
    LH-RH 0
    PACAP-38 (Ovine) 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
Standard Curve:

Exaggerated anorexigenic response to restraint stress in A(y) mice is associated with elevated CRFR2 mRNA expression in the hypothalamus.
Bazhan NM, Yakovleva TV, Kazantseva AY, Makarova EN. Physiol Behav. 2013;120:19-25.

The effect of urocortin I on the hypothalamic ACTH secretagogues and its impact on the hypothalamic-pituitary-adrenal axis.
Bagosi Z, Csabafi K, Palotai M, et al. Neuropeptides. 2014;48(1):15-20.

Serotonin 2C receptor antagonism ameliorates novelty-induced hypophagia in aged mice.
Nahata M, Muto S, Nakagawa K, et al. Psychoneuroendocrinology. 2013;38(10):2051-64.

Fasting hyperglycemia in the Goto-Kakizaki rat is dependent on corticosterone: a confounding variable in rodent models of type 2 diabetes.
Beddow SA, Samuel VT. Dis Model Mech. 2012;5(5):681-5.

Suppression of the HPA axis during extrahepatic biliary obstruction induces cholangiocyte proliferation in the rat.
Quinn M, Ueno Y, Pae HY, et al. Am J Physiol Gastrointest Liver Physiol. 2012;302(1):G182-93.

Paired box 6 (PAX6) regulates glucose metabolism via proinsulin processing mediated by prohormone convertase 1/3 (PC1/3).
Wen JH, Chen YY, Song SJ, et al. Diabetologia. 2009;52(3):504-13.

The single-prolonged stress paradigm alters both the morphology and stress response of magnocellular vasopressin neurons.
Yoshii T, Sakamoto H, Kawasaki M, et al. Neuroscience. 2008;156(3):466-74.

Dietary sodium manipulation during critical periods in development sensitize adult offspring to amphetamines.
Mcbride SM, Culver B, Flynn FW. Am J Physiol Regul Integr Comp Physiol. 2008;295(3):R899-905.

Aquaporin 1 is important for maintaining secretory granule biogenesis in endocrine cells.
Arnaoutova I, Cawley NX, Patel N, Kim T, Rathod T, Loh YP. Mol Endocrinol. 2008;22(8):1924-34.

Effect of chronic stress on gastric emptying and plasma ghrelin levels in rats.
Ochi M, Tominaga K, Tanaka F, et al. Life Sci. 2008;82(15-16):862-8.

Neither intravenous nor intracerebroventricular administration of obestatin affects the secretion of GH, PRL, TSH and ACTH in rats.
Yamamoto D, Ikeshita N, Daito R, et al. Regul Pept. 2007;138(2-3):141-4.

Molecular aspects of adrenal regulation for circadian glucocorticoid synthesis by chronic voluntary exercise.
Otawa M, Arai H, Atomi Y. Life Sci. 2007;80(8):725-31.

Regulation of adrenal glucocorticoid synthesis by interleukin-10: a preponderance of IL-10 receptor in the adrenal zona fasciculata.
Koldzic-zivanovic N, Tu H, Juelich TL, et al. Brain Behav Immun. 2006;20(5):460-8.

Up-regulation of the expression of cocaine and amphetamine-regulated transcript peptide by electroacupuncture in the arcuate nucleus of diet-induced obese rats.
Tian DR, Li XD, Wang F, et al. Neurosci Lett. 2005;383(1-2):17-21.

Alterations in stress-associated behaviors and neurochemical markers in adult rats after neonatal short-lasting local inflammatory insult.
Anseloni VC, He F, Novikova SI, et al. Neuroscience. 2005;131(3):635-45.

Involvement of arginine vasopressin in endogenous central histamine-induced reversal of critical haemorrhagic hypotension in rats.
Jochem J. Inflamm Res. 2004;53(7):269-76.