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Corticotropin Releasing Factor (CRF) (Human, Rat, Mouse, Canine, Feline) - EIA Kit

Quantity:

Catalog #
Size
Price
EK-019-06 96 wells $503

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • Human Plasma: 2.7 ng/ml
    Human Plasma (extracted): 0.4 ng/ml
    Human Serum: 2.5 ng/ml
    Human Serum (extracted): 0.35 ng/ml
    Rat Plasma: 1 ng/ml
    Rat Serum (extracted): 0.06 ng/ml
  • Sample Extraction:
  • Recommended
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 0.33 ng/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 0.33 - 3.73 ng/ml
  • Cross Reactivity:
  • Peptide %
    CRF (Human, Rat) 100
    CRF (Ovine) 0
    CRF (Bovine) 0
    Prepro-CRF (125-151) (Human) 0
    Sauvagine (Frog) 0
    PACAP-38 (Human, Rat, Ovine) 0
    LH-RH 0
    ACTH (Human) 0
    [Arg8]-Vasopressin 0
    BNP-45 (Rat) 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
  • MSDS:
  • View/Download(PDF)
Standard Curve:

Captodiamine, a putative antidepressant, enhances hypothalamic BDNF expression in vivo by synergistic 5-HT2c receptor antagonism and sigma-1 receptor agonism.
Ring RM, Regan CM. J Psychopharmacol (Oxford). 2013;27(10):930-9.

Increased severity of dyspeptic symptoms related to mental stress is associated with sympathetic hyperactivity and enhanced endocrine response in patients with postprandial distress syndrome.
De giorgi F, Sarnelli G, Cirillo C, et al. Neurogastroenterol Motil. 2013;25(1):31-8.e2-3.

Ghrelin alters postnatal endocrine secretion and behavior in mouse offspring.
Kodomari I, Maruoka T, Yamauchi R, Wada E, Wada K. Neurochem Int. 2009;54(3-4):222-8.

Loss of cannabinoid receptor CB1 induces preterm birth.
Wang H, Xie H, Dey SK. PLoS ONE. 2008;3(10):e3320.

Suppression and recovery of the hypothalamic function after high-dose corticosteroid treatment in preterm infants.
Ng PC, Lam CW, Lee CH, Chan IH, Wong SP, Fok TF. Neonatology. 2008;94(3):170-5.

Gastrointestinal dysfunction induced by early weaning is attenuated by delayed weaning and mast cell blockade in pigs.
Moeser AJ, Ryan KA, Nighot PK, Blikslager AT. Am J Physiol Gastrointest Liver Physiol. 2007;293(2):G413-21.

Stress maladjustment in the pathoetiology of ulcerative colitis.
Kuroki T, Ohta A, Aoki Y, et al. J Gastroenterol. 2007;42(7):522-7.

Brain orexins and wake regulation in rats exposed to maternal deprivation.
Feng P, Vurbic D, Wu Z, Strohl KP. Brain Res. 2007;1154:163-72.

Expression of molecular equivalent of hypothalamic-pituitary-adrenal axis in adult retinal pigment epithelium.
Zmijewski MA, Sharma RK, Slominski AT. J Endocrinol. 2007;193(1):157-69.

CRH mediates inflammation induced by lipopolysaccharide in human adult epidermal keratinocytes.
Zbytek B, Slominski AT. J Invest Dermatol. 2007;127(3):730-2.

Stress signaling pathways activated by weaning mediate intestinal dysfunction in the pig.
Moeser AJ, Klok CV, Ryan KA, et al. Am J Physiol Gastrointest Liver Physiol. 2007;292(1):G173-81.

Investigation of the corticotropin-releasing hormone-proopiomelanocortin axis in various skin tumours.
Kim MH, Cho D, Kim HJ, et al. Br J Dermatol. 2006;155(5):910-5.

Characterization of a ultraviolet B-induced corticotropin-releasing hormone-proopiomelanocortin system in human melanocytes.
Zbytek B, Wortsman J, Slominski A. Mol Endocrinol. 2006;20(10):2539-47.

Metformin restores leptin sensitivity in high-fat-fed obese rats with leptin resistance.
Kim YW, Kim JY, Park YH, et al. Diabetes. 2006;55(3):716-24.

Alterations in stress-associated behaviors and neurochemical markers in adult rats after neonatal short-lasting local inflammatory insult.
Anseloni VC, He F, Novikova SI, et al. Neuroscience. 2005;131(3):635-45.

Corticotropin-releasing hormone and its structurally related urocortin are synthesized and secreted by human mast cells.
Kempuraj D, Papadopoulou NG, Lytinas M, et al. Endocrinology. 2004;145(1):43-8.

High levels of intrauterine corticotropin-releasing hormone, urocortin, tryptase, and interleukin-8 in spontaneous abortions.
Madhappan B, Kempuraj D, Christodoulou S, et al. Endocrinology. 2003;144(6):2285-90.

Regulation of 15-hydroxy prostaglandin dehydrogenase by corticotrophin-releasing hormone through a calcium-dependent pathway in human chorion trophoblast cells.
Mckeown KJ, Challis JR. J Clin Endocrinol Metab. 2003;88(4):1737-41.