Apelin-12 (Human, Rat, Mouse, Bovine) - EIA Kit


Catalog #
EK-057-23 96 wells $610

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Sample Extraction:
  • Recommended
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 0.07 ng/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 0.07 - 0.79 ng/ml
  • Cross Reactivity:
  • Peptide %
    Apelin-12 (Human) 100
    Apelin-13 (Human) 100
    Apelin-13 (Rat) 100
    Apelin-36 (Human) 100
    Apelin-36 (Rat) 100
    Alpha-ANP (Human) 0
    ANP (25-56) (N-ANP) 0
    ADM-52 (Human) 0
    BNP-32 (Human) 0
    CNP-22 0
    Ghrelin (Human) 0
    ET-1 (Human) 0
    Bradykinin 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
Standard Curve:

Serum levels of asymmetric dimethylarginine and apelin as potential markers of vascular endothelial dysfunction in early rheumatoid arthritis.
Di franco M, Spinelli FR, Metere A, et al. Mediators Inflamm. 2012;2012:347268.

Prenatal fasudil exposure alleviates fetal growth but programs hyperphagia and overweight in the adult male rat.
Butruille L, Mayeur S, Duparc T, et al. Eur J Pharmacol. 2012;689(1-3):278-84.

LDL-cholesterol lowering increases plasma apelin in isolated hypercholesterolemia.
Tasci I, Erdem G, Ozgur G, et al. Atherosclerosis. 2009;204(1):222-8.

Apelin stimulates glucose utilization in normal and obese insulin-resistant mice.
Dray C, Knauf C, Daviaud D, et al. Cell Metab. 2008;8(5):437-45.

The transcriptional co-activator PGC-1alpha up regulates apelin in human and mouse adipocytes.
Mazzucotelli A, Ribet C, Castan-laurell I, et al. Regul Pept. 2008;150(1-3):33-7.

Vascular effects of apelin in vivo in man.
Japp AG, Cruden NL, Amer DA, et al. J Am Coll Cardiol. 2008;52(11):908-13.

Circulating apelin concentrations in mother/infant pairs at term.
Malamitsi-puchner A, Gourgiotis D, Boutsikou M, Baka S, Hassiakos D, Briana DD. Acta Paediatr. 2007;96(12):1751-4.

HIF-1 regulates hypoxia- and insulin-induced expression of apelin in adipocytes.
Glassford AJ, Yue P, Sheikh AY, et al. Am J Physiol Endocrinol Metab. 2007;293(6):E1590-6.

Requirement of apelin-apelin receptor system for oxidative stress-linked atherosclerosis.
Hashimoto T, Kihara M, Imai N, et al. Am J Pathol. 2007;171(5):1705-12.

Cardiac resynchronization therapy increases plasma levels of the endogenous inotrope apelin.
Francia P, Salvati A, Balla C, et al. Eur J Heart Fail. 2007;9(3):306-9.

Reduced plasma apelin levels in patients with autistic spectrum disorder.
Boso M, Emanuele E, Politi P, et al. Arch Med Res. 2007;38(1):70-4.

Evidence for decreased circulating apelin beyond heart involvement in uremic cardiomyopathy.
Codognotto M, Piccoli A, Zaninotto M, et al. Am J Nephrol. 2007;27(1):1-6.

Down-regulation of cardiac apelin system in hypertrophied and failing hearts: Possible role of angiotensin II-angiotensin type 1 receptor system.
Iwanaga Y, Kihara Y, Takenaka H, Kita T. J Mol Cell Cardiol. 2006;41(5):798-806.

Reduced apelin levels in lone atrial fibrillation.
Ellinor PT, Low AF, Macrae CA. Eur Heart J. 2006;27(2):222-6.

Apelin, a newly identified adipokine up-regulated by insulin and obesity.
Boucher J, Masri B, Daviaud D, et al. Endocrinology. 2005;146(4):1764-71.

Novel role for the potent endogenous inotrope apelin in human cardiac dysfunction.
Chen MM, Ashley EA, Deng DX, et al. Circulation. 2003;108(12):1432-9.