740Y-P, is a cell-permeable phosphopeptide which activates PI 3-kinase, binding with high affinity to its p85 subunit. Activation of PI 3-kinase in turn inhibits PTEN, an important target in infertility drug discovery.

Sequence of 740Y-P: Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Lys-Lys-Met-Lys-Trp-Lys-Lys-Ser-Asp-Gly-Gly-pTyr-Met-Asp-Met-Ser (Modifications: Tyr-25 = pTyr)

	Activation of the PI3K-Akt-Foxo3 pathway in oocytes and increased follicle development after treatment with bpV(pic) and 740Y-P. (A) Nuclear exclusion of Foxo3 in oocytes of primordial follicles at 6h after treatment with bpV(pic) and 740Y-P.
	Activation of dormant primordial follicles after in vitro treatment with bpV(pic) and 740Y-P, followed by transplantation into the kidney capsule of ovariectomized hosts. (A) Ovaries at 14 days after transplantation. Isolated ovaries for control vs. bpV(pic), control vs 740Y-P, and control vs bpV(pic) plus 740Y-P group. (Scale bars:1 mm.)
	Retrieval of mature mouse oocytes for epigenetic analyses, in vitro fertilization, and early embryonic development. (A) Histology of ovaries with or without hCG treatment at 18 days after transplantation after pretreatment with bpV(pic) and 740Y-P.
	Increases in PIP3 by activation of PI3K or inactivation of PTEN antagonises Sema3A-induced growth cone collapse. (A) Chick DRG explants were cultured overnight in the presence of NGF. Prior to application of Sema3A-Fc at 1 µg/ml, the cultures were incubated with the PI3K-activating 740Y-P peptide at 40 µg/ml for 30 minutes, with the 740Y-P peptide and LY294002 (10 µM), or with the Y-P-peptide 1309 (at 40 µg/ml).