PAP or ABC: 1:500
||Reconstitute with 50 µl of distilled water for the equivalent of undiluted antiserum.|
||Please store the lyophilized antibody at -20°C upon receipt for up to 24 months. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.|
||Each vial contains 50 µl of lyophilized rabbit antiserum.|
Transient early food restriction leads to hypothalamic changes in the long-lived crowded litter female mice.
Sadagurski M, Landeryou T, Cady G, Bartke A, Bernal-mizrachi E, Miller RA. Physiol Rep. 2015;3(4)
Neonatal insulin action impairs hypothalamic neurocircuit formation in response to maternal high-fat feeding.
Vogt MC, Paeger L, Hess S, et al. Cell. 2014;156(3):495-509.
Adolescent binge-like ethanol exposure reduces basal α-MSH expression in the hypothalamus and the amygdala of adult rats.
Lerma-cabrera JM, Carvajal F, Alcaraz-iborra M, et al. Pharmacol Biochem Behav. 2013;110:66-74.
AMPK is essential for energy homeostasis regulation and glucose sensing by POMC and AgRP neurons.
Claret M, Smith MA, Batterham RL, et al. J Clin Invest. 2007;117(8):2325-36.
NPY/AgRP neurons are not essential for feeding responses to glucoprivation.
Luquet S, Phillips CT, Palmiter RD. Peptides. 2007;28(2):214-25.
Down-regulated expression of agouti-related protein (AGRP) mRNA in the hypothalamic arcuate nucleus of hyperphagic and obese tub/tub mice.
Bäckberg M, Madjid N, Ogren SO, Meister B. Brain Res Mol Brain Res. 2004;125(1-2):129-39.
Distribution of galanin-like peptide in the rat brain.
Takatsu Y, Matsumoto H, Ohtaki T, et al. Endocrinology. 2001;142(4):1626-34.
alpha-Melanocyte-stimulating hormone is contained in nerve terminals innervating thyrotropin-releasing hormone-synthesizing neurons in the hypothalamic paraventricular nucleus and prevents fasting-induced suppression of prothyrotropin-releasing hormone gene expression.
Fekete C, Légrádi G, Mihály E, et al. J Neurosci. 2000;20(4):1550-8.
Agouti-related protein containing nerve terminals innervate thyrotropin-releasing hormone neurons in the hypothalamic paraventricular nucleus.
Légrádi G, Lechan RM. Endocrinology. 1999;140(8):3643-52.